fig1

Figure 1. Expression of m3C-related molecules in HCC and their effects on HCC cell lines. (A) Relative mRNA expression levels of six m3C regulators in 16 pairs of HCC and adjacent tissues measured by qRT-PCR; (B) CCK8 assay assessing HCC cell proliferation following m3C regulator knockdown or overexpression; (C) Wound healing assay evaluating changes in cell migration ability after overexpression or downregulation of m3C regulators. (**** P < 0.0001, *** P <0.001, ** P <0.01, * P < 0.05, ns: not significant, P > 0.05). HCC: Hepatocellular carcinoma; m3C: 3-methylcytidine; qRT-PCR: quantitative real-time PCR.