fig5

Figure 5. Association between ANKRD6 expression and pathway activity in COAD. (A) KEGG pathway intersection analysis showing the relationship between ANKRD6 expression levels and tissue type. (B) GO-BP pathway intersection analysis showing the relationship between ANKRD6 expression levels and tissue type. (C) GSEA showing suppression of the KEGG_WNT_SIGNALING_PATHWAY in the high ANKRD6 expression group, with a negative normalized enrichment score (NES = -1.424) and statistically significant adjusted P-values and FDR. (D) Violin plots depicting GSVA score differences for intersecting pathways in normal tissues (blue) vs. tumor tissues (red) and high ANKRD6 expression (red) vs. low ANKRD6 expression (blue) groups. (E) Correlation analysis between ANKRD6 expression levels and intersecting pathway activity. The color scale denotes ANKRD6 expression, where red corresponds to higher levels and blue to lower levels. (***P < 0.001).