fig1

Figure 1. Bcl-2 family expression levels in A549 and SW1573 cells; silencing of Bcl-xL enhances TRAIL-induced cell death. (A) Expression levels of pro- and anti-apoptotic Bcl-2 family proteins as determined by Western blot analysis in H460, A549 and SW1573 cells. (B) Knockdown of Bcl-xL with a specific siRNA sensitizes for TRAIL-induced apoptosis in A549 and SW1573 cells. Values are means ± SEM of at least three separate experiments. Statistics: (A) BID, SW1573 vs. H460 and A549, P < 0.01; BAK, A549 and SW1573 vs. H460, P < 0.0.05; Bcl-2: A549 and SW1573 vs. H460: P < 0.001; Bcl-xL, A549 and SW1573 vs. H460, P < 0.01; (B) ^#P < 0.005 and *P < 0.05, compared to untreated.