fig4

Figure 4. H&E, Masson trichrome, and DCF staining of the myocardium in experimental mice treated with RAGE inhibitor FPS-ZM1 for 4 weeks. (A) H&E images (representative); (B) Quantification of H&E area; (C) Masson trichrome image (representative); (D) Pooled data of Masson trichrome staining; (E) DCF staining images (first row: DCF staining; second row: DAPI staining; third row: Merged images); (F) Pooled DCF intensity. Mean ± SEM, n = 6 mice per group, normal distribution was evaluated using the Shapiro-Wilk test, experimental results were evaluated using one-way ANOVA and then Tukey’s post hoc test, ^*P < 0.05 between labeled groups. H&E: Hematoxylin and eosin; DCF: dichlorofluorofluorescein; DAPI: 4’,6-diamidino-2-phenylindole; SEM: standard error of the mean; ANOVA: analysis of variance.