fig7

Figure 7. The effect of ATF5 overexpression or knockdown on AML12 cells. (A) Validation of ATF5 knockdown cell model by qPCR; (B) Validation of ATF5 overexpression cell model by qPCR analysis; (C) Nile Red staining showing PA-induced lipid accumulation in AML12 cells; (D) Measurement of TG content in AML12 cells under different treatments; (E) qPCR analysis of the expression levels of ATF5, HSP60, and LONP1 in the ATF5 overexpression model; (F) TMRE staining relative fluorescence intensity. (****P < 0.0001, ***P < 0.001, **P < 0.01, and*P < 0.05.) ATF5: Transcription factor 5; siATF5: small interfering RNA targeting transcription factor 5; OE-ATF5: transcription factor 5 overexpression; CON: control; PA: palmitic acid; AML12: alpha mouse liver 12; qPCR: TG: triglyceride; HSP60: heat shock protein 60; LONP1: Lon protease 1.