fig4

Figure 4. CircGNB1 mediates YH’s protective effects in diabetic nephropathy. (A) Verification of circGNB1 knockdown efficiency; (B) Cell viability analysis following siRNA transfection; (C-E) Inflammatory marker expression after circGNB1 depletion in YH-treated, HG-induced cells; (F-H) Oxidative stress indicators in circGNB1-silenced conditions; (I-L) Cell proliferation (Scale bar = 20 μm) and migration assessments (Scale bar = 100 μm) following circGNB1 knockdown. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. YH: Yohimbine; HG: high glucose; circGNB1: circular RNA GNB1; siRNA: small interfering RNA; sh-NC: short hairpin RNA negative control.