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Figure 1. LINC01133 is upregulated and shows a mitochondria-enriched distribution in A549/DDP cells. (A) The viability of A549 and A549/DDP cells after treatment with different doses of cisplatin (0, 1, 2, 4, 8, 16, and 32 μg/mL) was measured by a CCK-8 assay; (B) Statistical analysis of the IC₅₀ values for A549 and A549/DDP cells; (C) Volcano plot analysis of differentially expressed mt-lncRNAs in A549 vs. A549/DDP cells with |log₂FC| ≥ 1 and adjusted P < 0.05 thresholds. Orange dots represent lncRNAs whose expression is significantly upregulated in A549/DDP cells, blue dots represent lncRNAs whose expression is significantly downregulated, and gray dots indicate genes whose expression is not significantly different; (D) Hierarchical clustering heatmap showing differentially expressed mt-lncRNAs between A549 and A549/DDP cells. Different colors indicate relative gene levels, with blue and red representing low and high expression, respectively; (E) Comparison of LINC01133 levels in the mitochondria of both cell lines based on transcriptome sequencing data; (F) Validation of relative LINC01133 expression in the mitochondria of both cell lines by qRT-PCR; (G) Subcellular localization of LINC01133 detected by FISH. Nuclei were stained with DAPI (blue), LINC01133 was visualized with a specific probe (green), and mitochondria were labeled with MitoTracker (red). Scale bar: 50 μm. Nonlinear regression analysis was conducted to fit the dose-response curves. Student’s t-test was adopted for comparing two-group means. ^***P < 0.001. DDP: Cisplatin; CCK-8: Cell Counting Kit-8; IC₅₀: half-maximal inhibitory concentration; mt-lncRNAs: mitochondria-associated long non-coding RNAs; qRT-PCR: quantitative reverse transcription-polymerase chain reaction; FISH: fluorescence in situ hybridization; DAPI: 4′,6-diamidino-2-phenylindole.