fig6

Figure 6. CA increased cisplatin sensitivity by regulating Gpx4. AGS-CR cells were transfected with recombinant plasmids of pcDNA-Gpx4 (1.5 µg) for 48 h, and the Gpx4 mRNA (A) and protein (B and C) levels were assessed using qRT-PCR and western blot, respectively; (D) AGS-CR cells were treated with cisplatin (35 µm), CA (5 µm), and pcDNA-Gpx4 and cell death was assessed using CCK-8; (E and F) AGS-CR cells were treated with cisplatin (35 µm) and CA (5 µm) in the presence or absence of Gpx4 overexpression and clone formation ability was assessed. All statistical analyses, except for panel D (analyzed using one-way ANOVA followed by Tukey’s post-hoc test), were performed using Student’s t-test (n = 3). **P < 0.01, ***P < 0.001. CA: Corosolic acid; Gpx4: glutathione peroxidase 4; qRT-PCR: quantitative reverse transcription polymerase chain reaction; CR: cisplatin-resistant; CCK-8: cell counting kit-8; ANOVA: analysis of variance.