fig5

Figure 5. CA decreased Gpx4 expression. After treatment with cisplatin (35 µm) alone, CA (5 µm) alone, or combination treatment with cisplatin (35 µm) and CA (5 µm) in AGS cells, Gpx4 mRNA (A) and protein (B and C) levels were assessed using qRT-PCR and western blot, respectively. qRT-PCR (D) and western blot (E and F) analyses of Gpx4 expression in AGS, AGS-CR, and CA-treated AGS-CR cells. All statistical analyses were assessed using one-way ANOVA followed by Tukey’s post-hoc test (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. CA: Corosolic acid; Gpx4: glutathione peroxidase 4; qRT-PCR: quantitative reverse transcription polymerase chain reaction; CR: cisplatin-resistant; ANOVA: analysis of variance.