fig4

Figure 4. High Gpx4 expression predicted a poor OS and DFS. (A) Gpx4 expression was analyzed in the TCGA-STAD database through the GEPIA tool. STAD tissues (n = 408) were displayed as red and normal tissues (n = 211) were displayed as grey. The GEPIA tool was applied to analyze the correlation of Gpx4 expression with OS (B) and DFS (C) in the TCGA-STAD database; (D) qRT-PCR analysis of Gpx4 mRNA level in 19 pairs of GC tissues and normal tissues. Statistical significance was assessed using Student’s t test (n = 19). *P < 0.05, **P < 0.01. Gpx4: Glutathione peroxidase 4; OS: overall survival; DFS: disease-free survival; TCGA-STAD: the cancer genome atlas-stomach adenocarcinoma; GEPIA: gene expression profiling interactive analysis; qRT-PCR: quantitative reverse transcription polymerase chain reaction.