fig3

Figure 3. CA triggered GC cell ferroptosis by inhibiting Gpx4 expression. AGS and MKN-45 cells were exposed to 5 µm of CA for 24 h, and the levels of iron (A), ROS (B), MDA (C), and GSH (D) were assessed using a commercial assay kit. AGS cells were treated with 5 µm of CA for 24 h, and the mRNA (E) and protein (F) levels of Gpx4, Ptgs2, Acsl4, Fth1, Nox1, and Slc7a11 were assessed using qRT-PCR and western blot, respectively. All statistical analyses were assessed using Student’s t test (n = 3). **P < 0.01, ***P < 0.001. CA: Corosolic acid; GC: gastric cancer; Gpx4: glutathione peroxidase 4; Ptgs2: prostaglandin-endoperoxide synthase 2; Fth1: ferritin heavy chain 1; Nox1: NADPH oxidase 1; Slc7a11: solute carrier family 7 member 11; qRT-PCR: quantitative reverse transcription polymerase chain reaction.