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Figure 2. Colony-forming ability of IGROV-1 and IGROV-1/Pt1 cells overexpressing USP14. (A) Western blot analysis of USP14 protein expression in IGROV-1 and IGROV-1/Pt1 cells stably transfected with lentiviral particles carrying USP14 or a lentiviral control (ctr) vector; (B) IGROV-1 and (C) IGROV-1/Pt1 cells were seeded in 6-well plates for colony-forming assays on plastic. Twenty-four hours after seeding, cells were treated with cisplatin and incubated for 10 days. IC₅₀ value is the concentration inhibiting cell growth by 50% (IGROV-1: lenti ctr vector, IC₅₀ = 0.2 ± 0.01 µM; USP14 vector, IC₅₀ = 0.203 ± 0.087 µM; IGROV-1/Pt1: lenti ctr vector, IC₅₀ = 1.43 ± 0.245 µM; USP14 vector, IC₅₀ = 2.79 ± 0.29 µM; one-sided KW_E P = 0.05). USP14: Ubiquitin-specific protease 14.