fig6
Figure 6. CF10/LV is effective at inducing Top1cc and γH2AX, a biomarker of DNA DSBs in both HCT116R and HCT116P cells, consistent with TS/Top1 dual targeting by CF10 in 5-FU/LV-resistant cells while 5-FU is relatively ineffective at causing DNA DSBs. Shown are merged images for DAPI (blue), γH2AX (green), and Top1cc (red) for the indicated cell lines and treatment (5-FU 10 mM, CF10 1 mM, LV 1 mM) for 24 h. Complete images are included in Supplementary Figures 11-16. Figure presentation software is Olympus Confocal Laser Scanning Microscope Fluoview FV3000 (RRID: SCR_017015). LV: Leucovorin; DSBs: double-strand breaks; TS: thymidylate synthase; 5-FU: 5-fluorouracil.
