fig4

Figure 4. Myc is elevated in 5-FU/LV-resistant HCT116^R cells, but CF10/LV decreases levels of Myc and the Myc-target ABCB5 at both the protein and mRNA levels. (A) Western blot for Myc with quantification of the Western blot image by densitometry shown in (B) and quantification of mRNA levels by RT-qPCR shown in (C). (D) Western blot for ABCB5 with quantification of the Western blot image by densitometry shown in (E) and quantification of mRNA levels by RT-qPCR shown in (F). ^*P < 0.03, ^**P < 0.002, ^***P < 0.0002, ^****P < 0.0001. Figure preparation software is GraphPad Prism (RRID: SCR_002798). 5-FU: 5-Fluorouracil; LV: leucovorin; RT-qPCR: reverse transcription quantitative polymerase chain reaction.