fig3

Figure 3. 5-FU/LV (10 mM, 1 mM) and CF10/LV (1 mM, 1 mM) treatment for 24 h increase TS at both the protein (A and B) and mRNA (C) levels in HCT116^R cells, but only CF10/LV promotes ternary complex formation (TSCC) and efficient inhibition of TS enzymatic activity (D). ^*P < 0.03; ^****P < 0.0001. Figure preparation software is GraphPad Prism (RRID: SCR_002798). 5-FU: 5-Fluorouracil; LV: leucovorin; TS: thymidylate synthase; TSCC: TS classic complex.