fig8

Figure 8. Mechanism effects of combined JQ1 and PD-0332991 treatment on EMT in pancreatic 70301 cells. (A) qPCR analysis of EMT-related gene expression in 70301 cells treated with PD-0332991, with or without JQ1; (B) qPCR analysis of invasion-associated gene expression in 70301 cells treated with PD-0332991, with or without JQ1; (C) Western blot analysis of EMT markers and Wnt/β-catenin signaling components following treatment with PD-0332991, JQ1, or their combination in 70301 cells; (D) Western blot analysis of TGFβ/Smad pathway inhibition by JQ1 in 70301 cells. Data are presented as mean ± SEM from three independent experiments. ^*P < 0.05, ^**P < 0.01, ^****P < 0.0001; ns: not significant. EMT: Epithelial-to-mesenchymal transition; qPCR: quantitative polymerase chain reaction; SEM: standard error of the mean.