fig6

Figure 6. Combined JQ1 and PD-0332991 synergistically suppress tumor growth, migration, and invasion. (A) The combination of JQ1 and PD-0332991 significantly suppressed tumor growth in 70301 cells; (B) Morphological changes in 70301 cells before and after JQ1 monotherapy. Scales bars, 100 μm; (C and D) Effects of PD-0332991, JQ1, and their combination on the migration of 70301 cells. Scales bars, 100 μm; (E) CI analysis of JQ1 and PD-0339221. Cell viability was measured using the CCK-8 assay after 72 h of treatment in 70301 cells. All data are presented as mean ± SEM from at least three independent experiments, with three randomly selected fields per experiment. ^*P < 0.05; ^***P < 0.001; ^****P < 0.0001; ns: not significant. CI: Combination index; SEM: standard error of the mean.