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Figure 1. mRNA and protein expression of CDK4/6 and their correlation with OS. Differential expression patterns of CDK4 and CDK6 in (A) GSE28735, (B) GSE62165, and (C) GSE62452 cohorts; (D) Association between CDK4/6 expression and OS in the TCGA cohort; (E) Representative immunohistochemical staining of CDK4 and CDK6 in tumor and adjacent non-tumor tissues. Scales bars, 100 μm; (F and G) Quantification of CDK4 and CDK6 in 79 paired tumor and adjacent non-tumor tissues; (H and I) Association of CDK4 and CDK6 protein expression with OS in our cohort; (J) Kaplan–Meier survival analysis comparing patients with high CDK4 and CDK6 expression (CDK4^high/6^high) vs. low expression (CDK4^low/6^low). Data are presented as mean ± SEM from three experiments. ^*P < 0.05. CDK4/6: Cyclin-dependent kinases 4 and 6; OS: overall survival; TCGA: The Cancer Genome Atlas; SEM: standard error of the mean.