fig5

Figure 5. Low expression of hsa-circ-0001030 is associated with chemoresistance and unfavorable prognosis in TSCC patients. (A) Representative HE staining images showing the histopathological morphology of TSCC and adjacent non-tumor tissues; (B) RNA FISH detection of hsa-circ-0001030 expression and subcellular localization in tumor and para-tumor tissues, showing lower cytoplasmic expression in tumor cells; (C) Classification of 88 TSCC patients into high and low hsa-circ-0001030 expression groups based on RNA FISH signal scores (cutoff = 2.46); (D) Comparison of hsa-circ-0001030 expression between the primary tumor group and the cisplatin-resistant tumor group, showing significantly lower expression in the resistant group; (E) Kaplan–Meier survival analysis demonstrating that low hsa-circ-0001030 expressing patients exhibit significantly worse overall survival compared with patients with high expression levels. ^***P < 0.001. TSCC: Tongue squamous cell carcinoma; HE: hematoxylin–eosin; FISH: fluorescence in situ hybridization; circRNA: circular RNA.