fig1

Figure 1. Characterization of EVs isolated by differential ultracentrifugation from the NSCLC cell line NCI-H460 and its multidrug resistant counterpart NCI-H460/R. (A) Representative TEM images of EVs (left: scale bar = 0.5 µM; right: scale bar = 200 nm). Results are representative of one experiment; (B) Particle size distribution and mean size of EVs, analyzed by NTA. Data are represented as mean ± SEM of at least three independent experiments; (C) EV markers detected by WB in EVs and respective cells. Data are represented as mean ± SEM of at least three independent experiments. EVs: Extracellular vesicles; NSCLC: non-small cell lung cancer; TEM: transmission electron microscopy; NTA: nanoparticle tracking analysis; SEM: standard error of the mean; WB: Western blotting.