fig4

Figure 4. Differential cytotoxicity of ADC payloads across cancer cell lines from diverse tissue origins. Heatmap showing AUC values from dose–response viability assays for a panel of ADC payload compounds tested across a large set of human cancer cell lines. Each row represents a unique cancer cell line, annotated by tissue of origin (colored bar on the right-hand side), and each column corresponds to an ADC payload compound, annotated by mechanism of action on the top of the heatmap. AUC values are color-coded, with red indicating higher AUC (lower sensitivity; more resistant) and blue indicating lower AUC (higher sensitivity; more cytotoxic effect). Clustering (both rows and columns) was performed using hierarchical methods based on compound response profiles. ADC: Antibody-drug conjugate; AUC: area under the curve; MOA: mechanism of action.