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Figure 2. KLF5 regulates cell cycle, platinum drug resistance, and apoptosis-related pathways in KRAS-mutant CRC. (A) qPCR analysis of knockdown efficiency for different si-KLF5 sequences, with si-KLF5#2 showing the highest silencing efficacy; (B) Western blot validation of KLF5 knockdown efficiency; (C-E) RNA-seq analysis of differentially expressed genes (adjusted P-value < 0.05) in control (SHNC) and KLF5-knockdown (SHKLF5) KRAS-mutant CRC cells; (F) GO pathway analysis revealing top-ranked pathways altered upon KLF5 knockdown in SW620 cells; (G) KEGG pathway analysis highlighting the most significantly enriched pathways following KLF5 knockdown in SW620 cells. Data represent the mean ± SD, Student’s t-test. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. KLF5: Krüppel-like factor 5; CRC: colorectal cancer; qPCR: quantitative polymerase chain reaction; siRNA: small interfering RNA; SHNC: short hairpin negative control; SHKLF5: short hairpin KLF5; RNA-seq: RNA sequencing; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; SD: standard deviation.