fig7

From: Super-enhancer inhibitors THZ2 and JQ1 reverse temozolomide resistance in glioblastoma by suppressing SE-driven SOX9 expression

Figure 7. SOX9 regulates drug sensitivity through effects on GBM cell migration and invasion. (A and B) mRNA and protein expression of SOX9 in U87MG and U87MG-R cells (n = 3); (C) CUT&RUN analysis showing genes enriched with H3K27ac in U87MG-R cells; (D) Protein levels of overexpressed SOX9 in U87MG cells; (E) Protein levels of silenced SOX9 in U87MG-R cells; (F) Transwell assays to detect the migration and invasion in OE-SOX9 and si-SOX9 cells (n = 3). Scale bars: 20 μM. Data are presented as mean ± SD from three independent experiments (n = 3). ^***P < 0.001. Statistical significance was assessed using Student’s t-test or one-way ANOVA. GBM: Glioblastoma; SD: standard deviation; ANOVA: analysis of variance.