fig8

Figure 8. (A) EMP1 mRNA Expression Post-shRNA Transduction. Bar graph representing the relative expression levels of EMP1 mRNA in J82 bladder cancer cells. Cells transduced with shRNA targeting EMP1 (sh-EMP1) exhibit a significant decrease in EMP1 mRNA levels compared to the negative control (NC) group, indicating effective knockdown; (B) Colony Formation Assay Post-EMP1 Knockdown. Representative images of colony formation assays in 6-well plates. J82 cells treated with sh-EMP1 show an increased number of colonies compared to the NC, suggesting an enhanced clonogenic potential upon EMP1 suppression.