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Figure 3. Common analysis of mouse models of bone metastases. In total, 1 × 105 4T1 or 5 × 105 MDA-MB-231 cells were injected into BALB/c or BALB/c nude mice, respectively. BALB/c mice were imaged two weeks after tumour cell injection and BALB/c nude mice imaged four weeks after tumour cell injection. Mice were culled for downstream analysis 24 h after imaging. (A) Bioluminescence imaging of tumours in the skeleton of BALB/c and BALB/c nude mice by IVIS (I) and florescence imaging of MDA-MB-231 tumours in the tibia of a BALB/c nude mouse by LightToolsTM. (B) Two-cross sections of longitudinal and cross-sectional micro-CT images (I) and whole leg X-ray images of tumour bearing bones from BALB/c nu/nu mice. (C) Two-dimensional 3 µM histological sections of tumour bearing tibiae from BALB/c mice following staining with Goldner’s trichrome (I) and H&E (II); tumour in bone is highlighted with a red outline. Data shown are previously unpublished images from the Ottewell laboratory.
