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Figure 2. Alterations in relative amounts of LC3-I and II by autophagy inducer and inhibitor in Western blotting. A reagent increases amounts of LC3-II in both upper and lower panels. However, a further increase in LC3-II by combination of the reagent and chloroquine indicate that the reagent is an autophagy inducer, but when the reagent is an inhibitor, amounts of LC3-II remains unchanged in combination of the reagent and chloroquine. LC3: light chain 3; CQ: chloroquine