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Figure 2. Applications of liquid biopsy in lymphomas. A: cell free DNA (cfDNA) can be selectively isolated and extracted from plasma samples. cfDNA may be analysed with the Cancer Personalized Profiling by Deep Sequencing (CAPP-Seq) method, that exploits magnetic probes that selectively capture and isolate specific genomic regions of interests, namely the target region. The target region is then subsequently sequenced by a Next-Generation-Sequencing (NGS) approach; B: consistently, cfDNA analysis on the liquid biopsy allows the genotyping of lymphomas and allows a better understanding of lymphoma pathogenesis; C: liquid biopsy mirrors the mutational landscape of the lymph node biopsy in most of cases and, in addition, allows the identification of mutations not identified in the tissue biopsy; D: the integration of imaging methods with the monitoring of the kinetics of the mutations identified by liquid biopsy on cfDNA may allow a more sensitive and specific prediction of patients’ outcome. The colored bars indicate the various levels of cfDNA drop during therapy. A higher drop in the cfDNA concentration (in green) may predict a favorable response to therapy, whereas a smaller drop may predict resistance to therapy