fig5

Figure 5. Western blot analysis of long-term protein expression of key EGFR signaling pathway molecules. Metastatic MDA-MB-231 breast cancer cells (BCCs) were co-cultured with embryonic stem cell (ESC)-microstrands for 48 h, followed by removing ESC-microstrands, replacing BCC media, and analyzing protein expression at 72 h and 120 h. Western blots for protein expression in metastatic MDA-MB-231 BCCs (CC: co-cultured with ESC-microstrands; E: co-cultured with empty microstrands; C: non-co-cultured control) (A); densitometry of expression of EGFR (B), precursor TGF-α (C), mature TGF-α (D), pEGFR (E), and pERK (F) (***P < 0.001; **P < 0.01; *P < 0.05)