fig4

Figure 4. Protein expression of key signaling pathway molecules in metastatic MDA-MB-231 breast cancer cells (BCCs) after co-culture with embryonic stem cell (ESC)-microstrands for 48 h in comparison to non-aggressive MCF7 BCCs and 3T3 fibroblasts. Western blots show reduced EGFR, β-catenin, and mature TGF-α protein expression in the co-cultured MDA-MB-231 BCCs (CC: co-cultured with ESC-microstrands; E: co-cultured with empty microstrands; C: non-co-cultured control) (A); densitometry of protein expression of EGFR (B); β-catenin (C); mature TGF-α (D); NKD2 (E) (***P < 0.001; **P < 0.01; and *P < 0.05)