fig3

Figure 3. Reversal of gene expression of metastatic MDA-MB-231 breast cancer cells (BCCs) after exposure to embryonic stem cell (ESC)-microstrands at the transcriptional level as revealed by qRT-PCR analysis. A: After 72 h, significantly decreased TGF-α and increased NKD2 gene expression in BCCs co-cultured with ESC-microstrands compared to both empty microstrand and non-co-cultured controls; B: after 72 h, EGFR and vimentin gene expression decreased significantly compared to both controls; C: after 48 h, β-catenin and GAPDH gene expression declined in BCCs co-cultured with ESC-microstrands compared to the both controls (****P < 0.0001; ***P < 0.001: **P < 0.01; *P < 0.05)