fig4

Figure 4. ICAM-1 is important for attachment and cytotoxicity of T cells to breast cancer. A: Labelled T cells attached to MDA-MB-231LN cells monolayer. AF1q significantly reduced the number of attached T cells to the monolayer. When AF1q expression was suppressed, the number of attached T cells was increased. The Bar graph represents the % adhesion cells of total cells; B: ICAM-1 blocking antibody significantly reduced the number of attached T cells to the monolayer; C: a 72 h cytotoxicity assay was performed in 24 well plates where co-cultured at 10:1 or 20:1 E:T ratio with MDA-MB-231LN cells