fig5

Figure 5. Coexpression of ADAMTS-1 and fibulin-1 abolishes mammosphere formation. Left: representative images of the mammospheres derived from MCF-7 transfectants in two consecutive generations. Control, MCF-7 cells transfected with an empty vector. Top row, mammosphere appearance before dissociation. Bottom row, mammosphere appearance 6 days after dissociation. Right: mammospheres were dissociated and passaged at a density of 20 cell/well in 96-well plates, and MFUs were counted and calculated as a percentage of the mammospheres formed from the number of cells seeded. White bar: 100 μm