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Figure 4. Coexpression of ADAMTS-1 and fibulin-1 reduces the proliferation of the breast cancer cell lines MCF-7 and MDA-MB-231. The proliferative state of breast cancer cell lines was measured by quantifying the proliferative nuclear marker Ki-67 by immunocytochemistry. Top Left: representative images of Ki-67 staining in the indicated MDA-MB-231 transfectants; bar, 10 μm. Top Right: quantification of Ki-67-positive nuclei (as a percentage of total nuclei labeled by DAPI staining). Bottom Left, representative images of Ki-67 staining in the indicated MCF-7 transfectants; bar, 10 μm. Bottom Right, quantification of Ki-67-positive nuclei (as a percentage of total nuclei labeled by DAPI staining)