fig2

Figure 2. In vitro hyperthermia stimulates IFN-γ and IL-2 production from T-cells stimulated with monoclonal antibodies against CD3 and CD28. Blood samples were collected and incubated at 37 °C and 39 °C for 2 h, then PBMCs were extracted and co-cultured with monoclonal antibodies against CD3 and CD28 to measure IFN-γ and IL-2 production levels in each supernatant. Results are shown as twofold over the control (37 °C) for the average of five separate donors, and expressed as mean ± SEM. Statistical differences from the control were evaluated using paired t-test. P < 0.05 was recognized as statistically significant^[42]. IFN: interferon; IL: interleukin; PBMCs: peripheral blood mononuclear cells