fig4

Figure 4. ZA suppresses microenvironmental factors activating NF-κB and HIF in bone metastatic lesions. A: WST-1 assay of PC-3/MRS-BLI treated with varied concentrations of ZA. n = 3, *P < 0.05; B: Relative NF-κB (top) and HIF (bottom) activities of PC-3/MRS-BLI treated with varied concentrations of ZA. n = 3; C: Diagram of the hypothetical mechanism for NF-κB and HIF activation in bone-metastasized cancer cells, and for ZA function suppressing this activation. ZA: Zoledronic acid; NF-κB: nuclear factor-kappa B; HIF: hypoxia-inducible factor; MRS-BLI: multiplexed reporter system for bioluminescent imaging