fig4

Figure 4. Determination of the optimal time and concentration for BCG infection in THP-1 human macrophages. (A-D) The optimal infection time and MOI were further validated by WB analysis, with an MOI of 8 and an infection time of 24 h identified as optimal. All data are presented as mean ± standard deviation. ** (P < 0.01), *** (P < 0.001), n = 3. LBP: Lipopolysaccharide-binding protein; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; BCG: Bacillus Calmette-Guérin; THP-1: WB: western blotting; MOI: multiplicity of infection.