fig5

Figure 5. Overview of the B. bifidum glycobiome compared to HBS and PBS. (A) shows the heatmap of the presence/absence of the CAZy enzyme families identified in the soft-core genome of B. bifidum and in the other HBS. Families with potential enzymatic activity towards carbohydrates present in HMO and the intestinal mucin layer are highlighted by a blue triangle or a blue circle, respectively; (B) shows the presence in PBS of the CAZy enzymatic functionalities absent in the other HBS. The green rectangle reports the PBS with CBM32 associated with different GH families. CAZy: Carbohydrate-Active enZYmes; GH: glycosyl hydrolases; HBS: human-associated bifidobacterial species; PBS: phylogenetically related B. bifidum species; HMO: human milk oligosaccharide; CBM32: carbohydrate-binding module 32.