fig4

Figure 4. Lachnospiraceae strains restore gut microbiota structure in IBS mice (A and B) Alpha diversity indices: Chao1, and Sobs (C) Beta diversity: PCoA; (D) LEfSe analysis (LDA score > 2.0, P < 0.05); (E) Correlation heatmap between metabolites and gut microbes; (F) Correlation heatmap between disease phenotypic factors and gut microbes. (A and B) Data are presented as mean ± SEM. Different letters indicate statistically significant differences among groups (one-way ANOVA followed by Tukey HSD post hoc test, P < 0.05); (E and F) Spearman correlation analysis with Benjamini-Hochberg FDR correction. * adjusted q < 0.05, ** adjusted q < 0.01, *** adjusted q < 0.001; all displayed correlations also meet |r| > 0.6. TNF-α: Tumor necrosis factor-α; IL-6: interleukin-6; IL-10: interleukin-10; SOD: superoxide dismutase; MPO: myeloperoxidase; MDA: malondialdehyde; ZO-1: zonula occludens-1; IBS: irritable bowel syndrome; PCoA: principal coordinate analysis; LEfSe: linear discriminant analysis effect size; LDA: linear discriminant analysis; SEM: standard error of the mean; ANOVA: analysis of variance; HSD: honest significant difference; FDR: false discovery rate.