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Figure 2. Lachnospiraceae strains differentially alleviate neurological abnormalities in IBS mice. (A) Representative movement trajectories of mice in the open field test; (B) Total distance moved in the open field; (C) Spontaneous alternation rate (%) in the Y-maze test; (D) Total number of arm entries in the Y-maze test; (E-G) Expression levels of markers involved in the 5-HT system (Tph2, Slc6a4, and Htr1a) in the prefrontal cortex; (H-K) Expression levels of factors involved in the GABA/Glu system (Gad1, Slc1a2, and Gabbr1) and the astrocytic marker GFAP in the prefrontal cortex. Note: Data are presented as mean ± SEM. Different letters indicate statistically significant differences among groups (one-way ANOVA followed by Tukey HSD post hoc test, P < 0.05). GFAP: Glial fibrillary acidic protein; IBS: irritable bowel syndrome; 5-HT: serotonin; SEM: standard error of the mean; ANOVA: analysis of variance; HSD: honest significant difference.