fig4

Figure 4. Individual bar graphs depicting the concentrations (ng/mL) of compounds in the Glu/Gln/GABA pathway in cell-free supernatant from control and MUC2-supplemented bioreactors as measured by targeted LC-MS/MS. Bar graphs represent the following compounds: (A) Glutamine; (B) Glutamate; and (C) GABA. Analyzed by student's t-test. P < 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), P ≤ 0.0001 (****). Each dot represents data from an individual bioreactor (n = 3) and each error bar represents the standard deviation. Student's t-test: P < 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***). Graphs generated using GraphPad Prism software. GABA: γ-aminobutyric acid; MUC2: Mucin 2; LC-MS/MS: liquid chromatography-tandem mass spectrometry.