fig2

Figure 2. Composition of the resistome in the upper respiratory tract of domestic cats determined by metagenomic sequencing. (A and B) ANOSIM evaluating the resistomes in the upper respiratory tract of domestic cats from different Chinese regions (A) and/or different living conditions (B); (C and D) Abundances of the resistomes in the upper respiratory tract of domestic cats from different Chinese regions (C) and/or different living conditions (D); (E and F) Heatmaps showing the abundances of genes conferring resistance to different antimicrobial classes in the upper respiratory tract of domestic cats from different Chinese regions (E) and/or different living conditions (F). Mann-Whitney U test was performed to compare the abundances between two groups, using pairwise multiple comparison adjustments according to the Benjamini-Hochberg procedure. The significance level was set at a P value of < 0.05 (*) and P > 0.05 is not marked. Different Chinese regions: NEC - Northeast China, NC - Northern China, EC - Eastern China, CC - Central China, SC - Southern China, NWC - Northwest China, SWC - Southwest China. Different living conditions: M - catteries, D - animal hospitals, L - stray bases. Different antimicrobial classes: AG - aminoglycosides, BL - β-lactams, FA - fusaric acids, FD - fusidanes, GP - glycopeptides, LS - lincosamides, MDR - multidrug resistant phenotype, ML - macrolides, NS - nucleosides, PA - phosphonic acids, PN - phenicols, PT - peptides, QL - quinolones, RM - rifamycins, SGS - streptogramins, SNM - sulfonamides, TCL - tetracycline, TMC - tetracenomycin, TTP - trimethoprim. ANOSIM: Analysis of similarities.