fig6

Figure 6. CRISPR/Cas9 delivery process using polymer nanoformulations. (A) CRISPR/Cas9 structure. The composition and mechanism of action of the CRISPR/Cas9 system, including gene insertion and deletion, were demonstrated. (B) Non-viral vectors and viral vectors. Non-viral vectors describe a method for delivering sgRNA and Cas9 protein into cells via electroporation and polymer NPs. Viral vectors describe three forms. (C) Nano-based CRISPR/Cas9 delivery. The process of delivering the CRISPR/Cas9 system into tumor cells by polymeric NPs was demonstrated. (D) Applications of polymeric NPs. The application of polymeric NPs to target specific gene mutations in different cancer types is demonstrated. Figure created by MEDGY. PEI: Polyethyleneimine; PAMAM: polyamidoamine; NPs: nanoparticles; sgRNA: single-guide RNA; EVs: extracellular vesicles; crRNA: CRISPR RNA; tracrRNA: trans-activating crRNA.