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Figure 5. Synthesis, characterization, and anti-tumor activity of PPH-siALK nanocomplexes. (A) Chemical synthesis of PPH. (B and C) PPH2k-siALK inhibited the malignant behavior of tumor cells. (D) An orthotopic ALK-positive non-small-cell lung cancer (NSCLC) xenograft model was established by tail vein injection of H3122 cells, with inhalation treatment initiated at Day 0. (E-H) Mice treated with inhaled PPH2k-siALK exhibited significantly slower tumor bioluminescent growth than control groups. (I) The proliferation activity of tumor cells in mice treated with PPH2k-siALK was reduced. Copyright 2025^[133]. PPH: pH-responsive charge-reversal polymer-siRNA complex; ALK: anaplastic lymphoma kinase. Significance thresholds were defined as follows: *P <0.05.