fig5

Figure 5. Effect of 6-PPDQ exposure on the expression of genes encoding potential retinoic acid receptors. (A) Effect of 6-PPDQ exposure on expressions of sex-1, nhr-1, nhr-10, nhr-35, nhr-64, and nhr-91. ^**P < 0.01 vs. control; (B) Effect of intestinal RNAi of alh-3 and dhs-19 on expression of sex-1 in 6-PPDQ-exposed nematodes. Exposure concentration of 6-PPDQ was 10 μg/L. ^**P < 0.01 vs. wild-type (L4440); (C) Effect of RNAi of sex-1 on intestinal expressions of pmk-1, bar-1, and daf-16 in 6-PPDQ-exposed nematodes. Thirty intact intestines were isolated for qRT-PCR analysis. Exposure concentration of 6-PPDQ was 10 μg/L.^**P < 0.01 vs. wild-type (L4440).6-PPDQ: N’-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone; RNAi: RNA interference; qRT-PCR: quantitative real-time polymerase chain reaction.