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Figure 5. Caproic acid mitigates AngII-induced senescence of MOVAS cells via the GPR41 receptor. (A) Schematic workflow of Gpr41 siRNA knockdown and AngII/CA intervention in MOVAS cells. (B) Representative western blot images showing the protein expression of GPR41, p53, p21 and p16 in different treatment groups. α-tubulin and GAPDH were used as internal loading controls. (C-F) Densitometric quantitative analysis of relative protein levels of GPR41 (C), p53 (D), p21 (E) and p16 (F). n = 4 biological replicates per group. Data are presented as mean ± SD. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s multiple comparison test. *P < 0.05, **P < 0.01; ns: Not significant; CA: caproic acid; AngII: angiotensin II; siNc: control siRNA; siGpr41: Gpr41-targeted siRNA. (A) was created in BioRender. Xie, Y. (2026) https://BioRender.com/yvqfe0r.