fig6

Figure 6. Macrophage STING promotes ZBP1-mediated NLRP3 inflammasome activation in cardiomyocytes. HL-1 was co-cultured with BMDMs for 24 h. HL-1 cells from each group (n = 3 biological replicates) were collected for RNA extraction using TRIzol reagent (Invitrogen), followed by RNA-seq. (A and B) Volcano plot, KEGG enrichment analysis from RNA-seq of HL-1 cells; (C) Heatmap analysis of inflammatory signaling pathway-related genes; (D and E) mRNA levels of ZBP1 in HL-1 cells co-cultured with BMDMs (n = 4 in each group); (F) Prediction of the interaction between ZBP1 and the NOD-like receptors signaling pathway from STRING. Mean ± SEM; *P < 0.05. STING: Stimulator of interferon genes; ZBP1: Z-DNA binding protein 1; BMDMs: bone marrow-derived macrophages; SEM: standard error of the mean; NLRP3: NOD-like receptor thermal protein domain associated protein 3; KEGG: Kyoto Encyclopedia of Genes and Genomes; NOD: nucleotide-binding oligomerization domain; STRING: Search Tool for the Retrieval of Interacting Genes/Proteins; TNF: tumor necrosis factor; NETF: neutrophil extracellular trap formation; JAK-STAT: Janus kinase-signal transducer and activator of transcription; VPI: viral protein interaction.