fig4

Figure 4. Macrophage STING knockout alleviates the hypertrophic effect of HL-1 cardiomyocytes co-cultured with cGAMP-treated macrophages. WT or Sting^-/- BMDMs were pretreated with or without cGAMP (1 μg/mL) for 15 min permeabilization. Then, HL-1 cells were co-cultured with BMDMs. (A-C) mRNA levels of ANP, BNP, and MYH7 in HL-1 cells co-cultured with WT or Sting^-/- BMDMs (n = 3 in each group); (D) Protein levels of ANP, BNP, and MYH7 in HL-1 cells co-cultured with WT or Sting^-/- BMDMs; (E and F) Cell surface area of HL-1 cells co-cultured with WT or Sting^-/- BMDMs (n = 3 in each group). Mean ± SEM; **P < 0.01, ***P < 0.001. One-way ANOVA with Bonferroni correction for 4A-C, 4F. STING: Stimulator of interferon genes; BMDMs: bone marrow-derived macrophages; mRNA: messenger RNA; SEM: standard error of the mean; cGAMP: cyclic GMP-AMP; WT: Wild-type; ANP: atrial natriuretic peptide; BNP: B-type natriuretic peptide; MYH7: myosin heavy chain 7; ANOVA: analysis of variance.