fig2

Figure 2. Gain-of-function mutation of STING in macrophage promotes cardiomyocyte hypertrophy in co-culture. (A) Sting^{N153S floxed} mice were crossed with LysM-Cre mice to render the myeloid STING N153S mice (Sting^{LysM N153S}). Then isolate the BMCs from bone marrow (generated by FigDraw, ID: UPTYU88837); (B) Protein and phosphorylated levels of IRF3 (Ser396) and TBK1 (Ser172) in BMDMs of Sting^{N153S floxed} and Sting^{LysM N153S} mice; (C) Schematic diagram of co-culture of HL-1 cells and Sting^{N153S floxed} and Sting^{LysM N153S} BMDMs (generated by FigDraw, ID: OITPS2a3a3); (D) Secreted IFN-β levels in medium of the above co-culture system (n = 4 in each group); (E-G) mRNA levels of ANP, BNP, and MYH7 in HL-1 cells co-cultured with Sting^{N153S floxed} and Sting^{LysM N153S} BMDMs (n = 4 in each group); (H and I) Cell surface area of HL-1 cells co-cultured with Sting^{N153S floxed} and Sting^{LysM N153S} BMDMs (n = 4 in each group). Mean ± SEM; *P < 0.05, **P < 0.01. Student’s t-test for 2D-G, 2I. STING: Stimulator of interferon genes; BMDMs: bone marrow-derived macrophages; mRNA: messenger RNA; SEM: standard error of the mean; BMCs: bone marrow cells; IRF3: interferon regulatory factor 3; TBK1: TANK binding kinase 1; IFN-β: interferon-β; ANP: atrial natriuretic peptide; BNP: B-type natriuretic peptide; MYH7: myosin heavy chain 7.