fig6

Figure 6. Impact of diet switch on CD45⁺ immune cells identified by scRNA-seq in the heart and spleen under homeostatic (no-MI) and infarcted (post-MI) conditions. (A) t-SNE plot of immune cells in LV of CON (10 months of lab chow), OBD (10 months of omega-6-enriched diet), and OBD-R (10 months of OBD diet + 4 months of CON diet) groups under homeostatic conditions (no-MI). Cell lineages are annotated by marker gene expression and color-coded; (B) t-SNE plot of immune cells in LV of CON, OBD, and OBD-R groups under infarcted conditions (post-MI-d1); (C) Fold-change graphs showing GOI (gene of interest) expression in different lineages in no-MI LV samples; (D) Fold-change graphs showing GOI expression in MI-d1 LV samples; (E) t-SNE plot of immune cells in spleens of CON, OBD, and OBD-R groups under homeostatic conditions (no-MI); (F) t-SNE plot of immune cells in the spleens of CON, OBD, and OBD-R groups under infarcted conditions (post-MI-d1); (G) Fold-change graphs showing GOI expression in no-MI spleen samples; (H) Fold-change graphs showing GOI expression in MI-d1 spleen samples. Cell lineages are color-coded. * indicates GOI expression exclusively increased in neutrophils of the OBD group. MI: Myocardial infarction; MI‐d1: 1 day after MI surgery; NK: natural killer; scRNA‐seq: single‐cell RNA sequencing; t-SNE: t-distributed stochastic neighbor embedding; LV: left ventricle; CON: control diet; OBD: obesogenic diet; OBD-R: obesogenic diet reversal; SEM: standard error of the mean.