fig1

Figure 1. Chronic obesogenic diet impairs cardiac strain, which is partially reversed by a control diet. (A) Experimental schematic of the study design; (B) Body weight (BW) at study endpoint; (C) LV (Left Ventricle)/BW ratio; (D) Spleen/BW ratio; (E) Long-axis B-mode echocardiography showing LV wall trace and segmental strain changes in CON (10 months of lab chow), OBD (10 months of omega-6-enriched diet), and OBD-R (10 months of OBD diet + 4 months of CON diet) at baseline; (F) Global longitudinal strain (GLS); (G) Fractional shortening (FS); (H) Hematoxylin and eosin (H&E) staining of LV (40×), spleen (10×; * denotes large WP area, # denotes small WP area), and adipose tissues (20×; blue * shows small adipocytes, red * shows large adipocytes). Crown-like structures in adipose tissue are marked with red brackets. LV and spleen insets are 2× magnification. Data are presented as mean ± SEM; n = 10-13 per group. Comparisons were performed using one-way ANOVA with Tukey’s post hoc test: *P < 0.05 vs. CON; # P < 0.05 vs. OBD; ns: non-significant. WP: White pulp; CON: control diet; OBD: obesogenic diet; OBD-R: obesogenic diet reversal.